Determination of the Mineral Content, Phytochemical Composition and Antimicrobial Properties of the Ethanolic Extract of Citrus sinesis on Some Clinical Microorganisms
Authors: *Udinyiwe, C.O. And Omoregie, A.E.
DOI Info: http://doi.org/10.5281/zenodo.15778267
ABSTRACT
Medicinal plants have been employed as therapeutic agents before modern medicines existed. This study was conducted to examine the mineral content, phytochemical composition and antimicrobial properties of the ethanolic extract of Citrus sinesis on some clinical microorganisms. The phytochemical analysis was conducted using the method of A.O.A.C. Agar well diffusion method was used to determine the antimicrobial potentials of the ethanolic extract of Citrus sinesis. The mineral content showed that potassium had the highest concentration with 7415.67±0.33 mg/ml, while the least was phosphorus 0.51±0.00 mg/ml. The phytochemicals present in the ethanolic extract of the Citrus sinesis were glycosides, saponins, phenols, alkaloids, eugenols, terpenoids, flavonoids, tannins and reducing sugars. It was observed that Staphylococcus aureus had the highest zone of inhibition of 20 mm, while Escherichia coli and Klebsiella spp. had the least zone of inhibition of 11 mm. The bactericidal concentration was 100 mg/ml for all the bacterial isolates. The research showed that Citrus sinesis possessed antibacterial abilities like other medicinal plants.
Affiliations: Department of Science Laboratory Technology, Faculty of Life Sciences, University of Benin, P.M.B 1154, Benin City, Edo State, Nigeria.
Keywords: Citrus Sinensis, Orange, Proximate, Phytochemical, Bacteria, Fungi
Published date: 2025/06/30
