Isolation and Identification of Dunaliella Species for the Production of Biocrude using Hydrothermal Liquefaction Technique: A Mini Review

Authors: Ikhazuangbe, P.M.O., Eboibi B.E, Amabogha, B., Orugba, H. And Agarri, S.E.

DOI Info: http://doi.org/10.5281/zenodo.12599317

ABSTRACT

Screening, isolation, identification and cultivation of microalgae for production of biocrude have been explored by many researchers. The different techniques for isolation (micropipette washing and streak-plating techniques) and culturing (convectional and novel techniques) used by these researchers were reviewed in this work. The elemental and biochemical composition of microalgae were reviewed, and Van Kreven chart was generated and compared for Dunaliella, Chlorella, Nannochloropsis and Spirullina species. The effects of light, oxygen content, temperature, carbon dioxide, nutrient concentration, salinity, mixing, and dilution rate, which affects the rate of growth of microalgae, composition of the Dunaliella microalgae biomass, molecular identification of the morphological characteristics was also reviewed. The various challenges and prospect encountered in the isolation and screening of pure microalgae species were evaluated. Despite numerous research investigations in this field, several challenges still exist including isolation and cultivation of pure species of Dunaliella for the commercialization of biocrude production from microalgae.

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