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Sensitivity Pattern of Some Commercial Antibiotics to Bacteria Isolated from Cotton Leaf Treated Well Water

Authors: Adetitun DO, Oguntoye MB, Kolawole OM


Water pollution occurs when harmful substances or microorganisms contaminate water bodies such as Rivers, Streams, Wells, and Lakes thereby reducing the quality of water and rendering it unfit for use in the environment or by humans. In this study, water samples were collected from a well five times and analyzed to determine the efficacy of commercial antibiotics on bacteria isolates obtained. A fifth sample was used as the control. Antimicrobial activities were observed against five (5) isolated bacteria: Escherichia coli, Klebsiella pneumonia, Shigella dysenteriae, Salmonella typhimurium and Proteus vulgaris. Physicochemical analysis, bacteriological analysis, biochemical tests and morphological identification of isolates were also carried out. From the results, the highest pH value of 6.9 was observed in the control, while the fourth sampling time had the lowest pH value of 3.5. Turbidity was highest; 0.564 NTU in the fourth sample with a contact time of 0 hrs and it was lowest (0.030 NTU) in the control sample with a contact time of 72 hrs. Total bacterial counts showed 105 × 104cfu/ml as the highest value for bacteriological analysis while 2 × 104 cfu/ml was the lowest value from both total coliform counts and total bacterial count in the bacteriological analysis. The highest inhibition zone of 30 mm was observed in Escherichia coli and Salmonella typhimurium on Amoxicillin and Chloramphenicol respectively while Chloramphenicol showed the lowest significant action with an inhibition value of 18 mm observed in Klebsiella pneumonia. Results of this study indicated the sensitivity pattern of some commercial antibiotics on isolates from well water.

Affiliations: Department of Microbiology, Faculty of Life Sciences, University of Ilorin, Nigeria
Keywords: Antibiotics, Sensitivity, Well Water, Pollution, Bacteria
Published date: 2019/06/30

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ISSN: 2635-3342 (Print)

ISSN: 2635-3350 (Online)

DOI: In progress

ISI Impact Factor: In progress

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Chemical Engineering Department, Faculty of Engineering, University of Benin, PMB 1154, Ugbowo, Benin City, Edo State, Nigeria.