Utilization of Hexadecane and Heptane by Serratia sp. under Different Potassium Nitrate (KNO3) Concentrations
Authors: Adetitun DO, Owoseni VO, Tomilayo RB
Composition of mineral salts medium vary depending on the scientific protocol used. Potassium nitrate (KNO3) is one of the components of mineral salts medium (MSM) used in microbiology and other experiment. In this work, the ability of Serratia sp. to degrade hexadecane and heptane at different KNO3 concentrations was studied. Four experiments were carried out with variations in hexadecane, heptane, salt concentrations and the days of observations. Mineral salts medium composed of 0.125 g/ml of KH2PO4, 0.35 g/ml of Na2HPO4, 0.05 g/ml of MgSO2.7H2O, 0.075 g/ml of KNO3 and 0.25 g/ml of (NH4)2SO4 was used. Growth rate using plate count method was assessed during the days of observation. Results showed the ability of Serratia sp. to degrade hexadecane and heptane as the only source of carbon and energy, because the total viable count increased from 79 x 102 to 172 x 106 cfu/ml for the normal MSN with hexadecane as carbon source. In the set up with normal MSM with heptane, total viable count increased from 22 x 102 to 452 x 106 cfu/ml. In the set up with hexadecane and 4 times KNO3 in normal MSM, the total viable count increased from 26 x 102 to 33 x 106 cfu/ml. In the fourth set up with heptane and 4 times KNO3 in MSM, the total viable count increased from 117 x 102 to 238 x 102 cfu/ml. It is concluded that the mixture of the hydrocarbons enhanced the degradation efficiency of Serratia sp.
Affiliations: Department of Microbiology, Faculty of Life Sciences, University of Ilorin, PMB 1515 Ilorin, Kwara State, Nigeria
Keywords: KNO3, Hexadecane, Heptane, Serratia Sp., Salts
Published date: 2019/12/30